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    CRL-1442 BRL 3A 大鼠肝細胞

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    產品名稱: CRL-1442 BRL 3A 大鼠肝細胞
    產品型號: CRL-1442
    產品廠商: 美國標準生物品收藏中心(ATCC)
    產品文檔: 無相關文檔


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    CRL-1442 BRL 3A 大鼠肝細胞,ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞;細胞庫管理規范,提供的細胞株背景清楚,提供參考文獻和*優培養條件!


    CRL-1442 BRL 3A 大鼠肝細胞 的詳細介紹

    CRL-1442 BRL 3A 大鼠肝細胞

    ATCC® Number:  CRL-1442™       
    Designations:  BRL 3A 
    Depositors:   SP Nissley, MM Rechler 
    Biosafety Level: 1 
    Shipped:  frozen 
    Medium & Serum:  See Propagation 
    Growth Properties: adherent
    Organism: Rattus norvegicus (rat) 
    Morphology: 
    CRL-1442 BRL 3A 大鼠肝細胞 
    Source: Organ: liver
    Strain: Buffalo
    Cellular Products: somatomedin like multiplication stimulating activity (MSA) 
    Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. 
     
    Comments: The serum-free conditioned medium from this cell line is a source of MSA. MSA is a family of polypeptides that can partially satisfy the serum reguirement of chick embryo fibroblasts. [1060]
    Propagation:  ATCC complete growth medium: Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%
    Atmosphere: air, 95%; carbon dioxide (CO2), 5%
    Temperature: 37.0°C
    Growth Conditions: For 24 hrs after initiating culture from a thawed ampule or after subculturing, grow the cells in culture medium with 5% fetal bovine serum. After 24 hrs, the cells may be transferred to serum-free medium.
    Subculturing:  Protocol: CRL-1442 BRL 3A 大鼠肝細胞

    Remove and discard culture medium.
    Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
    Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
    Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
    To remove trypsin-EDTA solution, transfer cell suspension to centrifuge tube and spin at approximately 125 xg for 5 to 10 minutes.Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels.
    Incubate cultures at 37°C.

    Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
    Medium Renewal: 2 times per week
    Preservation:  Freeze medium: Complete growth medium supplemented with 10% fetal bovine serum and 5% DMSO
    Storage temperature: liquid nitrogen vapor phase
    Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
    References: 1060: Nissley SP, et al. Proliferation of buffalo rat liver cells in serum-free medium does not depend upon multiplication-stimulating activity (MSA). Cell 11: 441-446, 1977. PubMed: 302146
    22582: Coon HG, Weiss MC. A quantitative comparison of formation of spontaneous and virus- produced viable hybrids. Proc. Natl. Acad. Sci. USA 62: 852-859, 1969. PubMed: 4308097
    22711: Dulak NC, Temin HM. A partially purified polypeptide fraction from rat liver cell conditioned medium with multiplication-stimulating activity for embryo fibroblasts. J. Cell. Physiol. 81: 153-170, 1973. PubMed: 4735141
    22712: Dulak NC, Shing YW. Large scale purification and further characterization of a rat liver cell conditioned medium multiplication stimulating activity. J. Cell. Physiol. 90: 127-130, 1977. PubMed: 833209
    22762: Schalch DS, et al. Nonsuppressible insulin-like activity (NSILA). I. Development of a new sensitive competitive protein-binding assay for determination of serum levels. J. Clin. Endocrinol. Metab. 46: 664-671, 1978. PubMed: 755052 
     
     CRL-1442 BRL 3A 大鼠肝細胞

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